Signalling

Part:BBa_K4044009:Design

Designed by: Kalinichenko Andrei   Group: iGEM21_LMSU   (2021-10-21)


BphP1-BcLOV4_QPAS1-Gal4 optogenetic system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 815
    Illegal PstI site found at 1112
    Illegal PstI site found at 1559
    Illegal PstI site found at 2608
    Illegal PstI site found at 2852
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 815
    Illegal PstI site found at 1112
    Illegal PstI site found at 1559
    Illegal PstI site found at 2608
    Illegal PstI site found at 2852
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
    Illegal BamHI site found at 1876
    Illegal BamHI site found at 4029
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 815
    Illegal PstI site found at 1112
    Illegal PstI site found at 1559
    Illegal PstI site found at 2608
    Illegal PstI site found at 2852
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 815
    Illegal PstI site found at 1112
    Illegal PstI site found at 1559
    Illegal PstI site found at 2608
    Illegal PstI site found at 2852
    Illegal NgoMIV site found at 457
    Illegal NgoMIV site found at 1208
    Illegal NgoMIV site found at 1705
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

none

References